My new collection of yeasts

Share your experiences of using brewing yeast.
Martin the fish

My new collection of yeasts

Post by Martin the fish » Thu May 15, 2008 12:10 pm

I have started to get myself a collection of yeasts. Mainly because my local HBS only sells S04 and often runs out. So i have to order from Aus if i want anything different-and put up with between 7 and 21 days delivery. All depending on how friendly MAF are feeling at the time.

So i now have, stored in my yeast fridge.

1/. 1968 ESB. 2nd generation-in 6 x PET's. (sorry SF :) )
2/. Ringwood. Wyeast smack pack. Split into 6 x PET's.
3/. Wyeast London Ale III. Smack pack.
4/. 2 x S04. Dried
5/. 2 x Nottingham. Dried
6/. 2 x Windsor. Dried


Eager to get trying these guys.

Who's got similar yeast 'hordes'? Mine is quite tiny so it'd be good to see some proper one's. I could probably get a pic of mine...

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Aleman
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Post by Aleman » Thu May 15, 2008 1:46 pm

I've trimmed mine down recently

Brewlabs Pilsner
Brewlabs High Gravity
Whitelabs Irish
Whitelabs Burton Ale
Whitelabs British Ale
Whitelabs Southern German Lager
Whitelabs German Bock Lager
Whitelabs 025 - A slant is labelled Essex Ale which means its the Adnams
Crouch Vale Brewery
Windsor
Nottingham
Brewferm Blanche
Saflager W34/70

Picture of the Effin brewery yeast bank :D

Image

JohnJeye

Post by JohnJeye » Thu May 15, 2008 2:18 pm

How do you work this then with these jars ?

I thought the idea with keeping yeasts in liquid form you just scoop the slurry up after a fermentation and keep it in the fridge in some kind of about 500ml container. Was all guess work though.

Also how long / how many uses can you get out of the yeasts this way. If its quite a few I would be more than happy to splash out the premium to get decent ones

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Post by Aleman » Thu May 15, 2008 2:45 pm

The yeast is stored under sterile distilled water in the jars . . . which is an excellent method for long term storage, as can be see from the dates on some of those Jars :). When I want to use the yeast, I shake to suspend the yeast in the vial, then heat a platinum loop in a flame until glowing, remove the lid of the vial, flaming the neck then working in the 'zone of the flame' I transfer a loopful to another vial containing sterile wort. this is then sealed and left in a warm place to get going . . Step up to 20ml once its working (24-36 hours), then to 200ml, then to 2L. If I want to ensure its viable, I will plate it on a wort agar plate in a petri dish, and select a colony to use after 3 days.

Long term viability is probably around 10 years or so, storing slurry under wort or distilled water is limited to around 6 months, but because the amount of yeast is so small and the qty of water is so big (And there are no nutrients in the water) then the yeast go into suspended animation until the conditions improve.

When I buy a new Yeast variety, the tube is tipped into a 5L starter and I remove a loopful from dregs of the tube and store it in the distilled water . .. that becomes my primary source, and I'm always working from the original generation of yeast.

The method does involve the knowledge of sterile transfer techniques and how to produce sterile containers (Sterile not sanitised!), but for maintaining a working yeast collection long term it is invaluble

Other techniques for yeast storage are available, this is not meant as a criticism of any other method :lol:

JohnJeye

Post by JohnJeye » Thu May 15, 2008 3:10 pm

sounds pro, will have to do some more reading up though I think before I'm ready to undertake your method.

My mums a bio chemist though so I reckon she might have some gear / dishes I could grab to have a bash with.

Whorst

Post by Whorst » Thu May 15, 2008 3:14 pm

I have a slant in my fridge called "British Microbrewery." I love that strain. Unfortunately, it's unavailable now. Would be interesting to know its origins.

steve_flack

Post by steve_flack » Thu May 15, 2008 3:20 pm

On slant I have

WY 1388 - Duvel
Crouch Vale
WLP-380 Hefe IV
WLP-565 Saison Dupont
WY-1275 Thames Valley
Hook Norton
Hop Back
Wyeast Scottish Ale
WY 1007 - German Ale
WY - Ringwood
Woodfordes/Charles Wells
WLP-026 Premium Bitter
Brewlabs No 9
Brewlabs Standard Ale
WLP-023 Burton Ale
WLP-500 Chimay

Dried

US-05, S-04, Windsor, W34-70, S-189, K-97

steve_flack

Post by steve_flack » Thu May 15, 2008 3:32 pm

Whorst wrote:I have a slant in my fridge called "British Microbrewery." I love that strain. Unfortunately, it's unavailable now. Would be interesting to know its origins.
That's an old Brewtek yeast - my understanding is that you can still get them from Brewing Science in the US.

http://www.brewingscience.com/

agentgonzo

Post by agentgonzo » Thu May 15, 2008 4:23 pm

I have about 3 packs of dried yeast in the freezer as a backup if I run out of live yeasts or they smell. S-04, Muntons Gold and Nottingham I think.

Then in the fridge I have my live yeast. My general procedure is:
Sanitise a jam-jar by boiling it for ~30mins and take it to the local micro-brewery. There, a nice chap called Malcolm rinses it with sterilising solution and fills with live yeast from his bucket-in-the-fridge. Take home and keep in the fridge. Use about half normally within a week for the first brew.

Then I just take a scoop of the slurry when racking off and either dump into the next brew if brewing the same day, or store in the fridge for up to a week and then put into the next brew. If keeping for more than 2-3 weeks I'll wash and store under boiled water in the fridge, but that hasn't happened yet. I'll keep this for probably about 3-6 months, then step up and use the second half of the initial yeast sample from the brewery and repeat. Then head back to the brewery once this is used up.

For speciality yeasts, like chimay/weizen etc, I culture directly from the bottle and use, but do not keep. The local tescos/morrisons has the beer in stock and it's just easier to culture, test then use and dispose of than try to keep it for the ~1year between speciality brews.

Do people think this is OK?

Whorst

Post by Whorst » Thu May 15, 2008 4:31 pm

Brewing Science don't sell to home brewers. Brewpubs and micros only. White Labs may have it in their bank. I'll pop 'em an email.

steve_flack

Post by steve_flack » Thu May 15, 2008 5:47 pm

agentgonzo wrote: For speciality yeasts, like chimay/weizen etc, I culture directly from the bottle and use, but do not keep. The local tescos/morrisons has the beer in stock and it's just easier to culture, test then use and dispose of than try to keep it for the ~1year between speciality brews.

Do people think this is OK?
Depends on the bottle. There's no guarantee the yeast in the bottle is either microbiologically clean or that it's the primary strain (Chimay is but Weizen may not be). If by 'culture' you mean plate out the yeast on a Petri dish and pick good clean yeast from that then fine, otherwise you're stuck with what's in the bottle. Although it's cheaper to buy a bottle and make a starter from it, I'd rather buy the yeast from a source I know for certain is clean.

Of course for some yeasts that isn't an option.

prodigal2

Post by prodigal2 » Thu May 15, 2008 7:04 pm

I have been warned that many a weizen bottle has lager yeast in it, and not the primary yeast :shock:

SiHoltye

Post by SiHoltye » Thu May 15, 2008 8:53 pm

I startered up some hop back yeast in the last 10 days.

From 2 bottles dregs into 150mls wort, then on to 1L. Didn't ferment past 1.020 in either case and the 1L smelt cidery after krausen had gone so I ditched.

Fullers has worked for me in the past, but it has to be good and I'd have to be confident or else bin it. Just not worth risking 5hrs work on dirty/broken yeast.

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Post by Aleman » Thu May 15, 2008 9:19 pm

prodigal2 wrote:I have been warned that many a weizen bottle has lager yeast in it, and not the primary yeast :shock:
Schneider Hefe is the primary strain . . . I've not had any luck culturing it, although I haven't tried now that I have the yeast ranching gear . . . . Hmmmm

agentgonzo

Post by agentgonzo » Fri May 16, 2008 10:40 am

Aleman wrote:
prodigal2 wrote:I have been warned that many a weizen bottle has lager yeast in it, and not the primary yeast :shock:
Schneider Hefe is the primary strain . . . I've not had any luck culturing it, although I haven't tried now that I have the yeast ranching gear . . . . Hmmmm
I cultivated it for a Hefeweizen at Christmas. Yeast was fine, but fermented it too warm as I didn't have temperature control and the yeast taste was overpowering.

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