Rinsing Yeast (in Pictures)

Share your experiences of using brewing yeast.
Wolfy

Re: Rinsing Yeast (in Pictures)

Post by Wolfy » Fri Sep 09, 2011 7:18 am

weiht wrote:I have a jar of rinsed wlp001 in the fridge for 2 mths, and I'm wondering how long can it last assuming I still do a step up starter every time? What is the rule of thumb for viability? 6 mths?
I've had washed yeast that was still viable after 6months or even 9months, however 12month old samples did not revive in a starter.
Likely it depends on the yeast strain and other factors, but you should be right for 6 months at least.

weiht

Re: Rinsing Yeast (in Pictures)

Post by weiht » Fri Sep 09, 2011 10:05 am

greenxpaddy wrote:Mr Malty says that your slurry is 50% viable. Best practice is to use a small amount of the slurry to step up from leaving the large quantity of dead cells in the jar. Keep stepping up until you have enough to pitch. For a standard size batch 19L you could use only 10ml of your thick slurry in a starter to get there - if using a stirplate. If not use twice the amount.
I used mr malty calculator and under the slurry tab, I got only 10% viability when I input harvest date at exactly 2 mths ago... Am I doing it right since u get 50%

greenxpaddy

Re: Rinsing Yeast (in Pictures)

Post by greenxpaddy » Fri Sep 09, 2011 1:14 pm

You're right - I was looking at the wrong tab...in which case you'd be best to do a two step starter

weiht

Re: Rinsing Yeast (in Pictures)

Post by weiht » Fri Sep 09, 2011 4:36 pm

Hmm at 10% viable yeast, I wonder if it's still ok to propagate and get good yeast or just buy a new pack... 10g into a 100ml starter n step up from there to 1.5l with stirplate?

greenxpaddy

Re: Rinsing Yeast (in Pictures)

Post by greenxpaddy » Fri Sep 09, 2011 4:42 pm

I would have no hesitation in doing that over buying new. amazing how the bleeders multiply. My slant from Tuesday is now a mass of yeast cells settling out in the fridge. I must have a good 75ml I reckon

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Jim
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Re: Rinsing Yeast (in Pictures)

Post by Jim » Wed Sep 14, 2011 4:23 pm

I've made this (very) useful thread sticky. Nice one yet again, Wolfy. 8)
"Reality is that which, when you stop believing in it, doesn't go away" - Philip K. Dick

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ead101

Re: Rinsing Yeast (in Pictures)

Post by ead101 » Wed Sep 14, 2011 4:38 pm

Wolfy + all the other Yeast forum regulars = Heroes of Legend

mrmojorisin

Re: Rinsing Yeast (in Pictures)

Post by mrmojorisin » Tue Oct 04, 2011 5:56 am

Many thanks wolfy, am about to do my first BIAB but was a bit concerned about the price of liquid yeast, if you can get 4 or 5 goes from 1 batch then buying wyeast isn't a problem.

dynamic dave

Re: Rinsing Yeast (in Pictures)

Post by dynamic dave » Mon Jan 02, 2012 8:32 pm

Has anyone used a sterile turkey baster to remove the top layer ?

Image

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Horatio
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Re: Rinsing Yeast (in Pictures)

Post by Horatio » Sat Feb 04, 2012 11:54 am

Can any tell me if Deionized Water is okay for yeast rinsing? I can't get Distilled locally but have a gallon on deionized? I know they are produced very differently to each other and wondered if the production method of deionized water would mean I would be best boiling it first?

Cheers all, :lol:
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guypettigrew
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Re: Rinsing Yeast (in Pictures)

Post by guypettigrew » Sat Feb 04, 2012 12:16 pm

Tap water seems to work OK for me!

Guy

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Horatio
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Re: Rinsing Yeast (in Pictures)

Post by Horatio » Sat Feb 04, 2012 12:36 pm

guypettigrew wrote:Tap water seems to work OK for me!

Guy
Cheers Guy. :lol:
If I had all the money I'd spent on brewing... I'd spend it on brewing!

De-Geert

Re: Rinsing Yeast (in Pictures)

Post by De-Geert » Sat Feb 04, 2012 9:58 pm

I would recommend not to use deionized water, that's not so good for the yeast cells. Tap water is better.

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Horatio
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Re: Rinsing Yeast (in Pictures)

Post by Horatio » Sat Feb 04, 2012 10:13 pm

De-Geert wrote:I would recommend not to use deionized water, that's not so good for the yeast cells. Tap water is better.
I thought I'd read that somewhere but wasn't sure. Thanks for the reply. :lol:
If I had all the money I'd spent on brewing... I'd spend it on brewing!

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Re: Rinsing Yeast (in Pictures)

Post by gregorach » Sun Feb 05, 2012 6:29 pm

I'd certainly recommend boiling any water you're going to use for rinsing.
Cheers

Dunc

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