Post
by McMullan » Mon Jan 29, 2018 6:32 pm
A number of home brewers, including myself, use microscopes to assess cell counts, as well as cell viability. The approximations are comparable to what most labs would report. It's a fairly straight forward procedure. It doesn’t have to be a precise measurement. It’s beer, not a life-threatening condition. As long as you have a ball-park number of healthy cells ( about 6 million per ml for a standard brew), you’re good to go. Being slightly under or over makes little difference, whatever the exact number might be for a given strain? Assuming sufficient healthy cells are pitched, it’s wort conditions that then matter. Temperature, of course, fermentability and and nutrients, especially oxygen. The final cell count in the fermenting wort is determined by wort resources, especially initial O2 levels, assuming sufficient cells were pitched. Personally, I recommend the use of yeast slants/slopes (BrewLabs in the UK). A 500ml starter produces sufficient cells for most brews. Make two starters if making a big beer or a lager. Harvesting and reusing yeast without making a starter, i.e. re-pitching within a few days, is fine, if using established techniques, e.g. top cropping or a variation of that, like IPA's method. However, fresh yeast are required after several harvests, to avoid the risk of losing a brew, after a long day preparing wort.